M. Mungai

Human Heart Failure Alters Mitochondria and Fiber 3D Structure Triggering Metabolic Shifts

This study identifies structural and metabolic changes in human heart failure using SBF-SEM and metabolomic analyses.

3D Mitochondrial Structure in Aging Human Skeletal Muscle: Insights into MFN-2 Mediated Changes

This study investigates age-related changes in mitochondrial 3D structure in human skeletal muscle and shows that exercise can restore mitochondrial integrity through mitofusin 2.

Quantitative Assessment of Morphological Changes in Lipid Droplets and Lipid-Mito Interactions with Aging in Brown Adipose

This study quantifies age-related changes in lipid droplet morphology and mitochondria-lipid interactions in murine brown adipose tissue using 3D imaging and analysis.

Components of Isolated Skeletal Muscle Differentiated Through Antibody Validation

The paper describes a method to isolate skeletal muscle from mice, generate satellite cells for culture, and validate cell populations using immunofluorescence.

Optimizing In Situ Proximity Ligation Assays for Mitochondria, ER, or MERC Markers in Skeletal Muscle Tissue and Cells

This paper describes a protocol for using proximity ligation assays (PLA) to study protein-protein interactions in mitochondria-endoplasmic reticulum contact sites (MERCs) in mouse skeletal muscle tissue and cells.

Creating Optimal Conditions for OPA1 isoforms by Western Blot in Muscle Tissue

This paper presents an optimized western blot protocol to isolate and detect five OPA1 isoforms in muscle tissue.

Ablation of Sam50 is associated with fragmentation and alterations in metabolism in murine and human myotubes

This study shows that Sam50 deficiency in myotubes leads to mitochondrial fragmentation, altered metabolism, and impaired oxidative capacity in both mice and humans.

Defining Mitochondrial Cristae Morphology Changes Induced by Aging in Brown Adipose Tissue

This study uses 3D electron microscopy to analyze mitochondrial cristae morphology in mouse brown adipose tissue across aging, finding significant structural changes in aged tissue.

ATF4 Dependent Increase in Mitochondrial-Endoplasmic Reticulum Tethering Following OPA1 Deletion in Skeletal Muscle

This study shows that OPA1 deficiency in skeletal muscle increases MERC formation via ATF4, affecting calcium exchange and mitochondrial-ER tethering.

3D Reconstruction of Murine Mitochondria Exhibits Changes in Structure Across Aging Linked to the MICOS Complex

This study examines age-related changes in mitochondrial 3D structure in mouse muscle and heart tissues, linking these changes to the MICOS complex and metabolic alterations.

Systematic Transmission Electron Microscopy-Based Identification and 3D Reconstruction of Cellular Degradation Machinery

This paper describes methods to identify and distinguish subcellular structures involved in macroautophagy using TEM and 3D reconstruction, with applications to quantify degradation machinery under different conditions.

Practices for Measuring 3D Organelle Morphology and Generating Surfaces with Amira

The paper introduces a standardized protocol using Amira software to quantify 3D organelle morphology from SBF-SEM data, demonstrating its application to mitochondria and ER structures.

A comprehensive approach for artifact-free sample preparation and assessment of mitochondrial morphology in tissue and cultured cells

This paper presents a method for preserving and quantitatively measuring mitochondrial morphology in tissues and cultured cells without introducing artifacts.

A Universal Approach to Analyzing Transmission Electron Microscopy with ImageJ

This paper introduces a standardized method using ImageJ to quantify subcellular organelle morphology in TEM images, focusing on mitochondria, ER, lysosomes, and autophagosomes.