Reprogramming Cas9 PAM Recognition for Allele-Specific Editing
Julia Amber Tartaglia, Vivian Nguyen, John J. Desmarais, Rachel F. Weissman, Brittney W. Thornton, Marena I. Trinidad, Kevin Briseno, Taylor R. Hudson, Carmelle Catamura, Liana F. Lareau, Fyodor D. Urnov, Jennifer A. Doudna, David F. Savage
This paper develops yeast-selected SpCas9 variants with re-specified PAM recognition to enable allele-specific editing, demonstrating improved specificity and catalytic efficiency compared to PAM-broadened variants.
bioRxiv · Aug 11, 2026 · PreprintMolecular Biology