Fyodor D. Urnov

This paper develops genome and epigenome editing tools to modulate TOX expression in human T cells and shows that TOX knockout has limited transcriptomic effects.

This paper develops yeast-selected SpCas9 variants with re-specified PAM recognition to enable allele-specific editing, demonstrating improved specificity and catalytic efficiency compared to PAM-broadened variants.

The paper uses an arrayed CRISPR screen to identify ZBTB10 as a regulator of human trophoblast differentiation, showing its role in fusion and hormone secretion.

The paper introduces dimelo-toolkit, a Python package for analyzing multimodal long-read epigenetic data, demonstrating its use with a novel DiMeLo-seq protocol to study chromatin state in GM12878 cells.

The paper identifies JCV VP1 epitopes recognized by HLA-A2 and isolates TCRs that recognize these epitopes, showing cytotoxic activity against infected cells.

The paper studies postreplicative immature chromatin in Sciara coprophila, finding that a specific locus remains in an immature state for 24 hours after replication, facilitating transcriptional access.

This study demonstrates precise control of gene expression levels by targeting the KRAB repressor domain in human cells.