Clear Native Gel Electrophoresis for the Purification of Fluorescently Labeled Membrane Proteins in Native Nanodiscs
Bence Ezsias, Nikolaus Goessweiner‐Mohr, Christine Siligan, Andreas Hörner, Carolyn Vargas, Sandro Keller, Peter Pohl
This paper introduces a method using charged polymer-encapsulated nanodiscs and FCS to purify fluorescently labeled membrane proteins in native-like lipid environments, avoiding aggregation and enabling structural studies.
Analytical Chemistry · Aug 1, 2025Peer-reviewedBiophysics