Engineering probiotic Escherichia coli Nissle 1917 to block transfer of multiple antibiotic resistance genes by exploiting a type I CRISPR-Cas system
Mengdie Fang, Ruiting Zhang, Chenyu Wang, Zhi-Zhi Liu, Mingyue Fei, Biao Tang, Hua Yang, Dongchang Sun
This paper engineers Escherichia coli Nissle 1917 to block antibiotic resistance gene transfer using a type I CRISPR-Cas system, showing efficacy in vitro and in zebrafish.
Applied and Environmental Microbiology · Sep 10, 2024Peer-reviewedMicrobiology