Chris Estell

Reduced non-specific binding of super-resolution DNA-PAINT markers by Shielded DNA-PAINT labeling protocols

The paper introduces Shielded DNA-PAINT, a method to reduce non-specific DNA-PAINT probe binding in cells, achieving a 5-fold reduction in non-specific events in the nucleus.

Human promoter directionality is determined by transcriptional initiation and the opposing activities of INTS11 and CDK9

The paper shows that sense transcription is more efficient than antisense initiation and that CDK9 and INTS11 opposingly maintain directionality in mammalian promoters.

ZC3H4 restricts non-coding transcription in human cells

This study identifies ZC3H4 as a factor that restricts non-coding transcription in human cells by promoting degradation of unstable transcripts, particularly antisense RNAs and super-enhancer transcripts.