Lorenzo Di Michele

This paper describes a DNA-based platform that spatially organizes and releases liposomes to mimic cellular communication.

The paper characterizes MINFLUX imaging performance over extended durations using DNA origami and corrects residual drift, achieving sub-2 nm precision without loss of site precision.

This paper designs and expresses non-natural membrane-less RNA organelles in E. coli, enabling algorithmic control over interactions and condensate structure.

This study creates synthetic DNA condensates that form single or two-phase structures, showing how their properties can be controlled and modeled using a Flory-Huggins framework.

This paper introduces a protein-free method for cyclic content exchange in synthetic cells using a hydrogel-core membrane architecture.

This study develops DNA-based receptors in synthetic membranes that respond to cations, enabling controlled assembly and catalytic activity, mimicking biological signaling mechanisms.

This paper demonstrates the localization of nucleic-acid cleaving enzyme activity within DNA-based membrane-less compartments, enabling spatially organized enzymatic reactions and dynamic pattern formation.

The paper introduces Shielded DNA-PAINT, a method to reduce non-specific DNA-PAINT probe binding in cells, achieving a 5-fold reduction in non-specific events in the nucleus.

This paper introduces a platform for engineering synthetic RNA condensates that can form programmable organelles with controlled properties.

This paper designs orthogonal RNA condensates using modular RNA motifs and demonstrates their ability to recruit proteins and form in living cells.

The paper designs a synthetic signaling network that mimics immune cell responses to bacterial metabolism through coordinated synthetic agents.

This paper introduces a platform for creating DNA-based artificial cells with localized functionalities using reaction-diffusion waves, demonstrating spatial segregation of RNA synthesis and storage.

The paper introduces Repeat DNA-PAINT, a method that reduces background and non-specific signals in DNA-PAINT by lowering imager concentration, while maintaining spatial resolution.

This paper introduces PD-PAINT, a method for sub-diffraction imaging of protein pairs with nanoscale resolution, and demonstrates its application to DNA-origami tiles and cardiac proteins.