Christopher Deich

A Chemically Defined Synthetic Cell Capable Of Growth And Replication

The paper describes a synthetic cell capable of growth, replication, and division using a 90kb genome encoding essential cellular functions.

One-pot cloning and protein expression platform for genetic engineering

This paper introduces a one-pot cloning and protein expression platform that integrates mutagenesis, plasmid assembly, and functional testing in a single reaction, validated with multiple protein systems.

Controlled exchange of protein and nucleic acid signals from and between synthetic minimal cells

This study demonstrates the controlled exchange of protein and nucleic acid signals between synthetic minimal cells using cell penetrating peptides.

A gene expression control technology for cell-free systems and synthetic cells via targeted gene silencing and transfection

The paper introduces a silencing oligo method to control gene expression in cell-free systems and synthetic cells through targeted mRNA binding and transfection.

New Aequorea fluorescent proteins for cell-free bioengineering

The paper validates new Aequorea fluorescent proteins for use in cell-free expression systems.

Parasites, infections and inoculation in synthetic minimal cells

This paper demonstrates a synthetic cell system that models host-parasite interactions, resistance to infection, and an inoculation system for immunity.

T7Max transcription system

The paper introduces a modified T7 RNA polymerase promoter, T7Max, that enhances transcription and translation yields in cell-free systems by increasing transcript concentration.

Expanding luciferase reporter systems for cell-free protein expression

This paper expands luciferase reporter systems for cell-free protein expression by introducing two orthogonal luciferases and a substrate regeneration pathway.

Akaby - cell-free protein expression system for linear templates

The paper introduces Akaby, a modified E. coli strain that improves protein expression from linear DNA templates in cell-free systems by reducing DNA degradation.