Joseph M. Heili

One-pot cloning and protein expression platform for genetic engineering

This paper introduces a one-pot cloning and protein expression platform that integrates mutagenesis, plasmid assembly, and functional testing in a single reaction, validated with multiple protein systems.

Controlled exchange of protein and nucleic acid signals from and between synthetic minimal cells

This study demonstrates the controlled exchange of protein and nucleic acid signals between synthetic minimal cells using cell penetrating peptides.

T7Max transcription system

The paper introduces a modified T7 RNA polymerase promoter, T7Max, that enhances transcription and translation yields in cell-free systems by increasing transcript concentration.

Differentiation of Pluripotent Synthetic Minimal Cells via Genetic Circuits and Programmable Mating

The paper demonstrates a method to engineer genetic circuits in synthetic minimal cells, enabling differentiation into new lineages through programmable mating or small molecule stimuli.