Judee Sharon

This paper introduces a one-pot cloning and protein expression platform that integrates mutagenesis, plasmid assembly, and functional testing in a single reaction, validated with multiple protein systems.

This study demonstrates the controlled exchange of protein and nucleic acid signals between synthetic minimal cells using cell penetrating peptides.

The paper introduces a modified T7 RNA polymerase promoter, T7Max, that enhances transcription and translation yields in cell-free systems by increasing transcript concentration.

The paper introduces Akaby, a modified E. coli strain that improves protein expression from linear DNA templates in cell-free systems by reducing DNA degradation.