Wakana Sato

This paper introduces a one-pot cloning and protein expression platform that integrates mutagenesis, plasmid assembly, and functional testing in a single reaction, validated with multiple protein systems.

The paper introduces a silencing oligo method to control gene expression in cell-free systems and synthetic cells through targeted mRNA binding and transfection.

The paper validates new Aequorea fluorescent proteins for use in cell-free expression systems.

The paper introduces a modified T7 RNA polymerase promoter, T7Max, that enhances transcription and translation yields in cell-free systems by increasing transcript concentration.

This paper expands luciferase reporter systems for cell-free protein expression by introducing two orthogonal luciferases and a substrate regeneration pathway.

The paper introduces Akaby, a modified E. coli strain that improves protein expression from linear DNA templates in cell-free systems by reducing DNA degradation.