Ancestors of Arylmalonate Decarboxylase show increased Activity, Stability and Stereoselectivity
Elske van der Pol, Jan Georg Gerstenberger, Xenia Georgiadou, Gyula Hoffka, Lisa-Marie Krammer, Klaus Peter Schliep, Colin Schür, Shina Caroline Lynn Kamerlin, Selin Kara, Robert Kourist
Ancestral sequence reconstruction of a bacterial decarboxylase gave enzymes with up to 294-fold longer half-life at 45 °C, higher activity, and 99.7% ee on a vinyl malonate substrate.
bioRxiv · Aug 20, 2026 · PreprintBiochemistry✦ In-depth digest