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Ancestors of Arylmalonate Decarboxylase show increased Activity, Stability and Stereoselectivity
Elske van der Pol, Jan Georg Gerstenberger, Xenia Georgiadou…·Robert Kourist
Ancestral sequence reconstruction of a bacterial decarboxylase gave enzymes with up to 294-fold longer half-life at 45 °C, higher activity, and 99.7% ee on a vinyl malonate substrate.