Author

D. G. Sashital

Listed by name: these papers carry no ORCID for this author, so different people with the same name may be mixed together.

Biology Disciplines

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Biochemistry· 5 Microbiology· 1

Signature Assays, Organisms & Methods

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Approach:experimental assay· 6 Research Focus:host-pathogen & immune response· 2 Organism:bacteria (other)· 1 Research Focus:cell type annotation· 1 Research Focus:chromatin & epigenetic regulation· 1 Assay:DNA methylation· 1 Research Focus:protein folding & dynamics· 1 Research Focus:protein structure prediction· 1

Frequent Co-Authors

A. J. Severin· 2 shared G. T. Nguyen· 2 shared K. Murugan· 2 shared M. A. Schelling· 2 shared Y. Dhingra· 2 shared A. S. Seetharam· 1 shared A. Seetharam· 1 shared A. Sritharan· 1 shared

Activity by Year

2019-2023 · peak 2/yr

Co-Author Collaboration Network

10 top collaborators · 12 mutual co-author links

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2 shared papersBiochemistry

A. J. Severin

Mutual co-authors in cluster

Author profile →

6 papers

Recent·Most cited

CRISPR-Cas12a exhibits metal-dependent specificity switching

G. T. Nguyen, M. A. Schelling, K. A. Buscher, A. Sritharan, D. G. Sashital

This study shows that Cas12a's specificity changes with Mg2+ concentration, affecting cleavage efficiency and phage escape routes in different cellular environments.

bioRxiv · Nov 29, 2023Biochemistry

Cas4/1 dual nuclease activities enable prespacer maturation and directional integration in a type I-G CRISPR-Cas system

Y. Dhingra, D. G. Sashital

This study identifies a type I-G CRISPR-Cas system where Cas4/1 fusion protein performs dual nuclease activities for prespacer maturation and directional integration.

bioRxiv · Jun 5, 2023Biochemistry

PAM binding ensures orientational integration during Cas4-Cas1-Cas2 mediated CRISPR adaptation

Y. Dhingra, S. K. Suresh, P. Juneja, D. G. Sashital

This study determines the cryo-EM structures of the Cas4-Cas1-Cas2 complex, revealing how PAM binding ensures correct spacer orientation during CRISPR adaptation.

bioRxiv · May 30, 2022Biochemistry

CRISPR-Cas effector specificity and target mismatches determine phage escape outcomes

M. A. Schelling, G. T. Nguyen, D. G. Sashital

This study shows that Cas12a mismatch tolerance and pre-existing target mismatches influence phage evolution, with liquid media leading to more phage escape despite in vitro cleavage activity.

bioRxiv · May 30, 2022 · PreprintMicrobiology

High-throughput in vitro specificity profiling of natural and high-fidelity CRISPR-Cas9 variants

K. Murugan, A. S. Seetharam, A. J. Severin, D. G. Sashital

This study compares cleavage activities and specificities of natural and engineered Cas9 variants using a high-throughput in vitro assay, revealing differences in off-target nicking and DSB formation.

bioRxiv · May 12, 2020 · PreprintBiochemistry

Pervasive off-target and double-stranded DNA nicking by CRISPR-Cas12a

K. Murugan, A. Seetharam, A. J. Severin, D. G. Sashital

This study investigates Cas12a cleavage specificity, revealing pervasive off-target nicking and double-stranded DNA degradation, which may impact its use in genome editing.

bioRxiv · Jun 2, 2019 · PreprintBiochemistry