Development of cell-free platforms for discovering, characterizing, and engineering post-translational modifications
Derek A. Wong, Zachary M. Shaver, Maria D. Cabezas, Martin Daniel-Ivad, Katherine F. Warfel, Deepali V. Prasanna, Sarah E. Sobol, Regina Fernandez, Robert Nicol, Matthew P. DeLisa, Emily P. Balskus, Ashty S. Karim, Michael C. Jewett
This paper develops a cell-free platform combining CFPS and AlphaLISA to rapidly study and engineer PTM installing proteins for therapeutic applications.
bioRxiv · Mar 25, 2024 · PreprintSynthetic Biology