Sarah E. Sobol

Development of cell-free platforms for discovering, characterizing, and engineering post-translational modifications

This paper develops a cell-free platform combining CFPS and AlphaLISA to rapidly study and engineer PTM installing proteins for therapeutic applications.

A low-cost recombinant glycoconjugate vaccine confers immunogenicity and protection against enterotoxigenic Escherichia coli infections in mice

This study develops a low-cost recombinant glycoconjugate vaccine against ETEC O148 and O78 that provides immunogenicity and protection in mice.

Cell-free expression and characterization of multivalent rhamnose-binding lectins using biolayer interferometry

This paper presents a cell-free expression method for multivalent rhamnose-binding lectins and demonstrates their direct analysis using biolayer interferometry for binding affinity and specificity.

A low-cost, thermostable, cell-free protein synthesis platform for on demand production of conjugate vaccines

This paper develops a low-cost, thermostable cell-free protein synthesis system for producing conjugate vaccines without refrigeration, achieving ~$0.50 per dose after 4 weeks at room temperature.