Katherine F. Warfel

Development of cell-free platforms for discovering, characterizing, and engineering post-translational modifications

This paper develops a cell-free platform combining CFPS and AlphaLISA to rapidly study and engineer PTM installing proteins for therapeutic applications.

A low-cost recombinant glycoconjugate vaccine confers immunogenicity and protection against enterotoxigenic Escherichia coli infections in mice

This study develops a low-cost recombinant glycoconjugate vaccine against ETEC O148 and O78 that provides immunogenicity and protection in mice.

Cell-free expression and characterization of multivalent rhamnose-binding lectins using biolayer interferometry

This paper presents a cell-free expression method for multivalent rhamnose-binding lectins and demonstrates their direct analysis using biolayer interferometry for binding affinity and specificity.

A low-cost, thermostable, cell-free protein synthesis platform for on demand production of conjugate vaccines

This paper develops a low-cost, thermostable cell-free protein synthesis system for producing conjugate vaccines without refrigeration, achieving ~$0.50 per dose after 4 weeks at room temperature.

Ribosome display of N-linked glycoproteins in cell-free extracts

This paper develops a method to produce N-linked glycoproteins using ribosome display in cell-free extracts, enabling their functional and structural analysis.

Improving cell-free glycoprotein synthesis by characterizing and enriching native membrane vesicles

The paper improves cell-free glycoprotein synthesis by characterizing and enriching native membrane vesicles in bacterial extracts to enhance membrane-bound functionality.

On-demand, cell-free biomanufacturing of conjugate vaccines at the point-of-care

This paper develops a portable, freeze-dried system for producing conjugate vaccines at the point-of-care, showing higher antibody responses in mice compared to traditional methods.