Intact protein barcoding enables one-shot identification of CRISPRi strains and their metabolic state
Vanessa Pahl, Paul Lubrano, Felicia Troßmann, Daniel Petras, Hannes Link
This paper introduces a method to simultaneously detect CRISPRi strain barcodes and metabolites using FI-MS, revealing pathway-specific metabolic changes in engineered bacteria.
Cell Reports Methods · Nov 26, 2024Peer-reviewedSynthetic Biology