Generation of clonal male and female mice through CRISPR/Cas9-mediated Y chromosome deletion in embryonic stem cells
Yiren Qin, Bokey Wong, Liangwen Zhong, Fuqiang Geng, Luis F. Parada, Duancheng Wen
This paper describes a method to generate clonal male and female mice using CRISPR/Cas9 to delete the Y chromosome in embryonic stem cells, enabling tetraploid complementation to produce viable mice.
The CRISPR Journal · Feb 1, 2021Peer-reviewedDevelopmental Biology