Interactomic affinity profiling by holdup assay: acetylation and distal residues impact the PDZome-binding specificity of PTEN phosphatase
P. Jane, G. Gogl, C. Kostmann, G. Bich, V. Girault, C. Caillet-Saguy, P. Eberling, R. Vincentelli, N. Wolff, G. Trave, Y. Nomine
The paper measures PDZ domain-binding affinity of PTEN PBM using holdup assay and fluorescence polarization, showing modulatory residues and acetylation affect specificity.
bioRxiv · Jul 1, 2020Biophysics