CReasPy-cloning: a method for simultaneous cloning and engineering of megabase-sized genomes in yeast using the CRISPR-Cas9 system
E. Ruiz, V. Talenton, M.-P. Dubrana, G. Guesdon, M. Lluch-Senar, F. Salin, P. Sirand-Pugnet, Y. Arfi, C. Lartigue
The paper introduces CReasPy-cloning, a method that uses CRISPR-Cas9 and yeast homologous recombination to clone and engineer bacterial genomes in yeast, demonstrating efficient multi-locus editing.
bioRxiv · May 29, 2019Bioengineering