P. Sirand-Pugnet

Comparative genomics of Mycoplasma feriruminatoris, a fast-growing pathogen of wild Caprinae

This paper compares the genomes of 14 Mycoplasma feriruminatoris strains to understand its genetic features and evolutionary relationships.

Evolution of the CRISPR-Cas9 defence system in Mycoplasma gallisepticum following colonization of a novel bird host

This study tracks the evolution of CRISPR-Cas9 in Mycoplasma gallisepticum after it shifted to a novel bird host, showing changes in spacers and PAM recognition, and a loss of CRISPR-Cas function over time.

Unblocking genome editing of major animal mycoplasmas using CRISPR/Cas9 base editor systems

This paper develops a CRISPR/Cas9 base editor system to efficiently edit the genomes of three major mycoplasma pathogens, enabling the study of virulence factors and vaccine development.

CReasPy-cloning: a method for simultaneous cloning and engineering of megabase-sized genomes in yeast using the CRISPR-Cas9 system

The paper introduces CReasPy-cloning, a method that uses CRISPR-Cas9 and yeast homologous recombination to clone and engineer bacterial genomes in yeast, demonstrating efficient multi-locus editing.

Removal of a subset of non-essential genes fully attenuates a highly virulent Mycoplasma strain

This paper attenuates a highly virulent Mycoplasma strain by deleting non-essential genes and demonstrates its safety in vivo using a goat model.