BioengineeringbioRxiv
CReasPy-cloning: a method for simultaneous cloning and engineering of megabase-sized genomes in yeast using the CRISPR-Cas9 system
E. Ruiz, V. Talenton, M.-P. Dubrana…·C. Lartigue
The paper introduces CReasPy-cloning, a method that uses CRISPR-Cas9 and yeast homologous recombination to clone and engineer bacterial genomes in yeast, demonstrating efficient multi-locus editing.
DOI 10.1021/acssynbio.9b00224