Author

Y. Arfi

Listed by name: these papers carry no ORCID for this author, so different people with the same name may be mixed together.

Biology Disciplines

Click to filter author papers

Microbiology· 4 Bioengineering· 1

Signature Assays, Organisms & Methods

Click to filter · or open tag

Assay:DNA methylation· 2 Research Focus:gene regulatory network inference· 2 Approach:genetic perturbation / CRISPR· 2 Research Focus:host-pathogen & immune response· 2 Organism:mouse· 2 Research Focus:cell type annotation· 1 Approach:experimental assay· 1 Research Focus:genome assembly· 1

Frequent Co-Authors

C. Lartigue· 4 shared P. Sirand-Pugnet· 4 shared A. Blanchard· 2 shared F. Rideau· 2 shared G. Gourgues· 2 shared T. Ipoutcha· 2 shared A. M. Liljander· 1 shared A. Villa· 1 shared

Activity by Year

2019-2023 · peak 2/yr

Co-Author Collaboration Network

10 top collaborators · 25 mutual co-author links

Click any collaborator node to inspect or filter shared papers

Focal author (center)Selected co-authorORCID co-authorUnlinked name

Curved chords = mutual co-authorship

4 shared papersMicrobiology

C. Lartigue

Mutual co-authors in cluster

Author profile →

5 papers

Recent·Most cited

Evolution of the CRISPR-Cas9 defence system in Mycoplasma gallisepticum following colonization of a novel bird host

T. Ipoutcha, I. Tsarmpopoulos, G. Gourgues, V. Baby, P. Dubos, G. E. Hill, Y. Arfi, C. Lartigue, P. Thebault, C. Bonneaud, P. Sirand-Pugnet

This study tracks the evolution of CRISPR-Cas9 in Mycoplasma gallisepticum after it shifted to a novel bird host, showing changes in spacers and PAM recognition, and a loss of CRISPR-Cas function over time.

bioRxiv · Mar 14, 2023 · PreprintMicrobiology

Unblocking genome editing of major animal mycoplasmas using CRISPR/Cas9 base editor systems

T. Ipoutcha, F. Rideau, G. Gourgues, Y. Arfi, C. Lartigue, A. Blanchard, P. Sirand-Pugnet

This paper develops a CRISPR/Cas9 base editor system to efficiently edit the genomes of three major mycoplasma pathogens, enabling the study of virulence factors and vaccine development.

bioRxiv · Mar 9, 2022 · PreprintMicrobiology

Imaging minimal bacteria at the nanoscale: a reliable and versatile process to perform Single Molecule Localization Microscopy in mycoplasmas

F. Rideau, A. Villa, P. Belzanne, E. Verdier, E. Hosy, Y. Arfi

This paper develops a reliable protocol for Single Molecule Localization Microscopy in mycoplasmas, enabling high-resolution imaging of subcellular structures.

bioRxiv · Feb 2, 2022Microbiology

CReasPy-cloning: a method for simultaneous cloning and engineering of megabase-sized genomes in yeast using the CRISPR-Cas9 system

E. Ruiz, V. Talenton, M.-P. Dubrana, G. Guesdon, M. Lluch-Senar, F. Salin, P. Sirand-Pugnet, Y. Arfi, C. Lartigue

The paper introduces CReasPy-cloning, a method that uses CRISPR-Cas9 and yeast homologous recombination to clone and engineer bacterial genomes in yeast, demonstrating efficient multi-locus editing.

bioRxiv · May 29, 2019Bioengineering

Removal of a subset of non-essential genes fully attenuates a highly virulent Mycoplasma strain

J. Jores, L. Ma, P. Ssajjakambwe, E. Schieck, A. M. Liljander, S. Chandran, M. H. Stoffel, V. L. Cippa, Y. Arfi, N. Asad-Garcia, L. Falquet, P. Sirand-Pugnet, A. Blanchard, C. Lartigue, H. Posthaus, , S. Vashee

This paper attenuates a highly virulent Mycoplasma strain by deleting non-essential genes and demonstrates its safety in vivo using a goat model.

bioRxiv · Jan 10, 2019Microbiology