Plant BiologybioRxiv
Impact of temperature and time on DNA-free Cas9-ribonucleoprotein mediated gene editing in wheat protoplasts and immature embryos
S. Poddar, J. Tanaka, K. L. D. Running…·B. Staskawicz
This study improves DNA-free Cas9-RNP gene editing in wheat by optimizing temperature and time, achieving efficient gene knockouts without exogenous DNA.
DOI 10.3389/fpls.2022.1084700