B. Staskawicz

The activated plant NRC4 immune receptor forms a hexameric resistosome

The study determines the cryo-EM structure of the auto-active NRC4 resistosome, revealing a hexameric configuration that triggers immune responses via cytosolic Ca2+ influx.

Editing cis-regulatory elements towards generating rice stomatal morphological variation for adaptation to broad and dynamic environments

This study edits cis-regulatory elements in rice to generate stomatal density variation, linking morphological changes to physiological traits and drought response.

Dosage differences in 12-OXOPHYTODIENOATE REDUCTASE genes modulate wheat primary root growth

This study identifies OPRIII genes in wheat that modulate root architecture and grain yield under water stress through jasmonic acid signaling.

Improvement of Gene Delivery and Mutation Efficiency in the CRISPR-Cas9 Wheat (Triticum aestivum L.) Genomics System via Biolistics

This study improves CRISPR-Cas9 mutation efficiency in wheat by optimizing gold particle size and heat treatment conditions.

Impact of temperature and time on DNA-free Cas9-ribonucleoprotein mediated gene editing in wheat protoplasts and immature embryos

This study improves DNA-free Cas9-RNP gene editing in wheat by optimizing temperature and time, achieving efficient gene knockouts without exogenous DNA.

Engineering of Sr33 and Sr50 plant immune receptors to alter recognition specificity and autoactivity

This study identifies amino acids in wheat NLRs Sr33 and Sr50 that mediate effector recognition and autoactivity, enabling engineered resistance.

A draft genome assembly for the heterozygous wild tomato Solanum habrochaites highlights haplotypic structural variations of intracellular immune receptors

This paper presents a draft genome assembly of Solanum habrochaites LA1353, revealing haplotypic structural variations that expand NLR gene inventory and enhance sequence evolution.

Screening of Cultivars for Tissue Culture Response and Establishment of Genetic Transformation in a High-yielding and Disease-resistant Cultivar of Theobroma cacao

The paper establishes tissue culture and genetic transformation methods for a high-yielding, disease-resistant Theobroma cacao cultivar and identifies factors improving T-DNA delivery and somatic embryogenesis.

Efficient isolation of protoplasts from rice calli with pause points and its application in transient gene expression and genome editing assays

This paper introduces a method for isolating rice protoplasts from calli with pause points, enabling flexible transfection and genome editing assays.

Evolution of NLR resistance genes with non-canonical N-terminal domains in wild tomato species

The paper analyzes NLR resistance genes in wild tomato species, revealing their evolutionary dynamics and structural diversity using SMRT sequencing.

High Aspect Ratio Nanomaterials Enable Delivery of Functional Genetic Material Without DNA Integration in Mature Plants

This study shows high aspect ratio nanomaterials can deliver functional genetic material to plants without DNA integration, using multiple species and demonstrating siRNA gene silencing.