Impact of temperature and time on DNA-free Cas9-ribonucleoprotein mediated gene editing in wheat protoplasts and immature embryos
S. Poddar, J. Tanaka, K. L. D. Running, G. K. Kariyawasam, J. Faris, T. Friesen, M.-J. Cho, J. H. Cate, B. Staskawicz
This study improves DNA-free Cas9-RNP gene editing in wheat by optimizing temperature and time, achieving efficient gene knockouts without exogenous DNA.
bioRxiv · Apr 5, 2022Plant Biology