J. H. Cate

A highly efficient human cell-free translation system

This paper presents an optimized human cell-free translation system that enhances mRNA translation efficiency by suppressing eIF2 phosphorylation through GADD34 and K3L overexpression.

Interactions between terminal ribosomal RNA helices stabilize the Escherichia coli large ribosomal subunit

The paper identifies RNA-RNA tertiary contacts stabilizing the E. coli 50S ribosomal subunit and shows how MS2 tags disrupt these interactions, proposing a solution to maintain stability.

Rare Ribosomal RNA Sequences from Archaea Stabilize the Bacterial Ribosome

This study identifies sequence variations in archaeal ribosomes that stabilize the ribosome at high temperatures and creates a stable mutant bacterial ribosome for engineering.

Impact of temperature and time on DNA-free Cas9-ribonucleoprotein mediated gene editing in wheat protoplasts and immature embryos

This study improves DNA-free Cas9-RNP gene editing in wheat by optimizing temperature and time, achieving efficient gene knockouts without exogenous DNA.

Huge and variable diversity of episymbiotic CPR bacteria and DPANN archaea in groundwater ecosystems

The paper characterizes CPR bacteria and DPANN archaea in groundwater ecosystems, finding high diversity and site-specific variation, with potential health implications.

Clades of huge phage from across Earth’s ecosystems

The paper identifies major clades of large phage genomes across diverse ecosystems and highlights their expanded genetic repertoires and potential functional roles.