Eukaryotic-driven directed evolution of Cas9 nucleases
G. V. Ruta, M. Ciciani, E. Kheir, M. D. Gentile, S. Amistadi, A. Casini, A. Cereseto
This study enhances weakly active Cas9 nucleases using a eukaryotic platform, resulting in a 12-fold increase in activity in mammalian cells while maintaining specificity.
bioRxiv · Sep 18, 2023Bioengineering