Synthetic BiologybioRxivPreprint
Structure-guided design of protein attachment points for functional augmentation of complex molecular machines
Georgie Hau Sørensen, Jessica James, Harrison Steel…·Thomas E. Gorochowski
This paper introduces SIMPLIFE, a computational workflow for designing optimal insertion sites for conjugation tags in proteins, demonstrating its use in augmenting T7 RNA polymerase function.
DOI 10.1101/2025.06.22.660945