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This paper engineers fluorescent orthogonal ribosomes to monitor synthetic protein production in bacteria, identifying an insertion site that enhances ribosome activity and enables dynamic tracking.

FPCountR calibrates fluorescence readings to absolute units using custom calibrants and novel assays, enabling accurate protein quantification in microbial systems.

This paper introduces SNAP as a quantitative reporter for bacterial cell display, enabling the development of a versatile platform for engineering low affinity ligands and enzymes.