High-Affinity Binding of Chemokine Analogs that Display Ligand Bias at the HIV-1 Co-receptor CCR5
C. A. Rico, Y. A. Berchiche, M. Horioka, J. C. Peeler, E. Lorenzen, H. Tian, M. A. Kazmi, A. Furstenberg, H. Gaertner, O. Hartley, T. P. Sakmar, T. Huber
The paper develops a fluorescence assay to study high-affinity chemokine analogs that show ligand bias at CCR5, finding they bind with subnanomolar affinities and occupy a site not accessible to native chemokines.
bioRxiv · Mar 16, 2019Biophysics