Mapping enzyme catalysis with metabolomic biosensing
L. Xu, K.-C. Chang, E. M. Payne, C. Modavi, L. Liu, C. Palmer, N. Tao, H. S. Alper, R. T. Kennedy, D. S. Cornett, A. R. Abate
The paper introduces a method to sense enzyme product formation using host cell metabolism and mass spectrometry to map enzyme activity and mutagenesis effects.
bioRxiv · Oct 15, 2021 · PreprintBioengineering