Precise tuning of gene expression output levels in mammalian cells
Yale S. Michaels, Mike Bogetofte Barnkob, Hector Barbosa, Toni A. Baeumler, Mary Kay Thompson, Violaine André, Huw Colin‐York, Marco Fritzsche, Uzi Gileadi, Hilary M. Sheppard, David J. H. F. Knapp, Thomas A. Milne, Vincenzo Cerundolo, Tudor A. Fulga
This paper introduces miSFITs, synthetic microRNA response elements that allow precise control of gene expression levels in mammalian cells, showing their utility in modulating antigen expression in a mouse melanoma model.
Nature Communications · Feb 18, 2019Peer-reviewedSynthetic Biology