David J. H. F. Knapp

Tumour-stroma signaling drives a reversible program with neural-like features in cancer-associated fibroblasts

The paper identifies tumor-derived signals that induce a reversible neural-like program in cancer-associated fibroblasts and shows it is regulated by growth factors and MAPK/ERK signaling.

Genome-wide structural variant landscape following HDR-enhanced CRISPR editing in human hematopoietic stem and progenitor cells

The paper investigates structural variant induction in human hematopoietic stem and progenitor cells during HDR-enhanced CRISPR editing, finding that SVs occur even without DNA-PK inhibitors and increase with multiple edits.

Spatial multi-omics resolve epithelium-fibroblast gradients and highlight NESTIN-NOTCH1-expressing subepithelial fibroblasts during human pancreatic tumorigenesis

This study uses spatial multi-omics to identify NESTIN-NOTCH1-expressing fibroblasts in IPMNs and their role in pancreatic tumorigenesis.

The Human Male Mammary Gland has Similar Epithelial Populations to Female but Distinct Composition and Transcriptional Properties

This paper presents a single-cell RNA sequencing atlas of the adult human male breast, revealing distinct transcriptional properties and composition compared to female breast tissue.

Cell State Chaos Underpins the Evolution of SMARCA4-Deficient Dedifferentiated Endometrial Cancer

This study shows that SMARCA4 loss in endometrial cancer leads to cell state chaos, not stemness, causing dedifferentiation through epigenomic dysregulation.

A Rare Multipotent Peg-like Epithelial Cell is a Candidate Cell-of-Origin for High-Grade Serous Ovarian Cancer

The paper identifies a rare multipotent epithelial cell in the fallopian tube as a potential cell-of-origin for high-grade serous ovarian cancer using multi-omic profiling and organoid models.

Receptor Tyrosine Kinase Profiling Identifies Chronic Constitutive Floodgate Oxidative Signaling in Glutathione-Independent Human Mammary Luminal Progenitor Cells

This study identifies cKIT as a key mediator of oxidative signaling in human mammary luminal progenitor cells and links its activation to poor outcomes in basal-like breast cancers.

A Digital Microfluidic Platform for the Microscale Production of Functional Immune Cell Therapies

This paper compares a digital microfluidic electroporation system (triDrop) with commercial systems for engineering human T cells, showing improved viability, efficiency, and reduced dysregulation of pathways.

Puberty Blocker and Aging Impact on Testicular Cell States and Function

The paper analyzes the impact of puberty blockers on testicular cell states and function using single-cell RNA sequencing and clinical data from PB-treated children.

Precise tuning of gene expression output levels in mammalian cells

This paper introduces miSFITs, synthetic microRNA response elements that allow precise control of gene expression levels in mammalian cells, showing their utility in modulating antigen expression in a mouse melanoma model.