Characterizing the portability of RecT-mediated oligonucleotide recombination
Gabriel T. Filsinger, Timothy M. Wannier, Felix Boel Pedersen, Isaac D. Lutz, Julie Zhang, Devon A. Stork, Anik Debnath, Kevin Gozzi, Helene Kuchwara, Verena Volf, Stan Wang, Xavier Rios, Christopher J. Gregg, Marc J. Lajoie, Seth L. Shipman, , George McDonald Church
This study investigates the portability of RecT-mediated homologous recombination across bacterial species, finding that compatibility with the host's single-stranded DNA-binding protein determines functionality.
bioRxiv · Apr 14, 2020 · PreprintSynthetic Biology