Marc J. Lajoie

A Rationally Designed Transgene Drives CAR T Functional Persistence and Durable Regression of Solid Tumors

This paper designs a transgene that enhances CAR T cell persistence and tumor regression by integrating multiple engineered technologies in solid tumor models.

A Dynamically Regulated Designed IL-2 for Tumor-localized Signaling

This paper designs a dynamically regulated IL-2 variant that enhances tumor-specific immune responses while reducing systemic toxicity.

Protein design to broadly reprogram engineered T cell function

This paper designs proteins to reprogram T cell signaling pathways, improving CAR-T and TCR-T performance in solid tumor models.

De novo design of modular and tunable allosteric biosensors

The paper designs modular biosensors using de novo protein switches that detect various analytes with high sensitivity and modularity.

Characterizing the portability of RecT-mediated oligonucleotide recombination

This study investigates the portability of RecT-mediated homologous recombination across bacterial species, finding that compatibility with the host's single-stranded DNA-binding protein determines functionality.

Optimizing complex phenotypes through model-guided multiplex genome engineering

The paper presents a method combining multiplex genome engineering and predictive modeling to identify minimal genomic modifications that optimize a complex phenotype in E. coli.

Millstone: Software for Multiplex Microbial Genome Analysis and Engineering

Millstone is a web-based platform for microbial genome analysis and engineering that automates genotype comparison and visualization for projects with up to hundreds of genomic samples.