John Aach

The paper shows recoded E. coli can escape faster due to tRNA base changes and introduces kill switches to prevent this.

This paper introduces a method for chromosome-scale haplotype-resolved genome assembly using long reads and long-range data, achieving high accuracy and contiguity in three human genomes.

This study investigates the portability of RecT-mediated homologous recombination across bacterial species, finding that compatibility with the host's single-stranded DNA-binding protein determines functionality.

The paper introduces a pedigree-graph-based method for haplotype-aware de novo assembly of related individuals using Illumina and PacBio data, achieving chromosomal-scale phased assemblies with low coverage.

The paper introduces BOLORAMIS, a RT-free method for spatially-resolved, targeted in-situ RNA detection, and demonstrates its use on human iPSCs to identify spatial localization and expression heterogeneity.

The paper presents a method combining multiplex genome engineering and predictive modeling to identify minimal genomic modifications that optimize a complex phenotype in E. coli.

This study demonstrates that Elongation Factor P facilitates the ribosomal incorporation of consecutive D-amino acids by resolving peptidyl transferase stalling.

This paper introduces targeted deaminases for site-specific C-to-T genomic edits without DNA cleavage, achieving 13% efficiency in E. coli and 2.5% in human cells with reduced toxicity.

CasFinder identifies specific Cas9 target sites in genomes with low off-targeting potential, generating exome-wide catalogs for human and mouse for three Cas9 variants.