Absolute Quantification of Fluorescent Protein Fusions by Proteomics
Anna Shevchenko, Archishman Ghosh, Andrea Schuhmann, Aliona I. Bogdanova, Henrik Thomas, Viditha Rao, Eric R. Geertsma, T.‐Y. Dora Tang, Andrej Shevchenko
The paper develops a mass-spectrometry-based method to absolutely quantify fluorescent protein fusions and estimate their mature chromophore fraction, validated through a case study on expression kinetics.
bioRxiv · Sep 19, 2025 · PreprintBiochemistry