Andrej Shevchenko

Identification of lipid specificity in membrane organizing protein complexes

The paper identifies lipid specificity in membrane organizing protein complexes by mapping lipid-protein interactions and demonstrating phosphatidylethanolamine's role in stabilizing NDC1 and MIC60.

Ribosomal frameshifting is a carefully tuned modifier of proteins

The paper identifies and characterizes ribosomal frameshifting sites across organisms, showing their prevalence and evolutionary tuning to mitigate harmful effects.

Absolute Quantification of Fluorescent Protein Fusions by Proteomics

The paper develops a mass-spectrometry-based method to absolutely quantify fluorescent protein fusions and estimate their mature chromophore fraction, validated through a case study on expression kinetics.

Quantitative imaging of species-specific lipid transport in mammalian cells

The paper quantifies lipid transport between organelles in mammalian cells, revealing non-vesicular transport as a key mechanism for species-specific lipid sorting.

Disruption of Plasma Membrane Lipid Asymmetry Alters Cellular Energetics

This study shows that disrupting plasma membrane lipid asymmetry in mammalian cells alters cellular energetics by shifting metabolism from anabolic to catabolic states.

Lipidome unsaturation affects the morphology and proteome of the Drosophila eye.

The paper investigates how dietary lipid manipulations affect the Drosophila eye proteome and identifies lipid-responsive proteins involved in muscle function and stress responses.

Hepatocyte differentiation requires anisotropic expansion of bile canaliculi

This study shows that hepatocyte differentiation requires anisotropic expansion of bile canaliculi, with loss of polarity leading to cholangiocyte-like gene expression.

Eye proteome of Drosophila melanogaster

The paper quantifies the proteome of the Drosophila melanogaster eye using GeLC-MS/MS and provides a resource for further studies.

Shedding light on biosilica morphogenesis by comparative analysis of the silica-associated proteomes from three diatom species

The paper identifies silica-associated proteins from three diatom species and finds that they lack commonality and show low sequence similarity, suggesting unique morphogenetic mechanisms.

Competition between histone and transcription factor binding regulates the onset of transcription in zebrafish embryos

Production of systemically circulating Hedgehog by the intestine couples nutrition to growth and development

This study shows that the intestine produces systemic Hedgehog in Drosophila larvae, linking nutrition to growth and developmental timing through hormonal signaling.

Lipoproteins carry endocannabinoids that inhibit the Hedgehog pathway

The paper identifies endocannabinoids in lipoproteins as inhibitors of the Hedgehog pathway in both flies and humans.