A Comprehensive Workflow for Imaging Live Insulin Secretion Events and Granules in Intact Islets
Margret A. Fye, Rahul Sharma, Phyu Sin M. Myat, P. H. Regan, Hudson McKinney, Guoqiang Gu, Irina Kaverina
This paper presents a method to image insulin secretion and granule dynamics in live pancreatic islets using Zn2+-binding dyes and TIRF microscopy, revealing distinct secretion behaviors of different insulin granule pools.
bioRxiv · Apr 22, 2025 · PreprintCell Biology