A Multiplexed Bacterial Two-Hybrid for Rapid Characterization of Protein-Protein Interactions and Iterative Protein Design
W. Clifford Boldridge, Ajasja Ljubetič, Hwangbeom Kim, Nathan B. Lubock, Dániel Szilágyi, Jonathan Lee, Andrej Brodnik, Roman Jerala, Sriram Kosuri
The paper develops a high-throughput method to characterize protein-protein interactions and design orthogonal coiled-coil pairs, identifying a large set of specific interactions.
Nature Communications · Aug 2, 2023Peer-reviewedMolecular Biology