Roman Jerala

Pharmacologically regulated bioorthogonal stabilization domain for regulation of CAR T cells

This paper develops a pharmacologically controlled system for regulating CAR T cell function using estrogen receptor-based degron domains and FDA-approved compounds.

Validation and analysis of 12,000 AI-driven CAR-T designs in the Bits to Binders competition

The paper validates and analyzes 12,000 AI-designed CAR-T binders from a global competition, identifying successful designs and factors improving their performance.

Hydroxychloroquine decouples reactogenicity from efficacy in mRNA and viral-vector gene delivery

This study shows hydroxychloroquine reduces inflammation from mRNA and viral-vector therapies without affecting their therapeutic effectiveness.

Disrupting Splicing Regulation to Rescue β-Catenin: A Novel Approach for Treating CTNNB1-Haploinsufficiency Disorder

This study uses spliceosome-mediated RNA trans-splicing to restore β-catenin production in CTNNB1 syndrome, showing improved trans-splicing efficiency with optimized PTMs.

Single-chain permuted proteins for dimerization-based control of protein activity and cellular processes

The paper introduces a method to control protein activity through dimerization-based conditional reconstitution, demonstrating its application in inducing cell death, virus detection, and immune cell stimulation.

A general strategy to engineer high-performance mammalian Whole-Cell Biosensors

This paper introduces a method to design high-performance mammalian whole-cell biosensors by integrating synthetic biology and control engineering, resulting in a versatile biosensor named CASense.

Designed allosteric protein logic

The paper introduces a system to regulate protein function and create Boolean logic gates using coiled-coil dimers in protein loops, demonstrated on ten diverse proteins.

Engineering a Scalable and Orthogonal Platform for Synthetic Communication in Mammalian Cells

This paper develops a scalable synthetic communication platform for mammalian cells using orthogonal receptors and coiled-coil peptides to enable logic gate computations in cell consortia.

A Multiplexed Bacterial Two-Hybrid for Rapid Characterization of Protein-Protein Interactions and Iterative Protein Design

The paper develops a high-throughput method to characterize protein-protein interactions and design orthogonal coiled-coil pairs, identifying a large set of specific interactions.

Regulation of protein secretion through chemical regulation of endoplasmic reticulum retention signal cleavage

The paper introduces two genetically encoded secretion systems that enable fast, chemically regulated protein secretion by cleaving ER retention signals, bypassing the need for transcription and translation.

Coiled-coil heterodimers with increased stability for cellular regulation and sensing SARS-CoV-2 spike protein-mediated cell fusion

This paper designs stable coiled-coil peptides for cellular regulation and detects SARS-CoV-2 spike protein-mediated cell fusion.