The eukaryotic replisome intrinsically generates asymmetric daughter chromatin fibers
Bruna V. Eckhardt, Hannah J. Richter, Inge Rondeel, Keerthi Renduchintala, Francesca Mattiroli, Vijay Ramani
The paper investigates chromatin asymmetry on newly replicated DNA strands using yeast replisome reconstitution and single-molecule chromatin footprinting, revealing intrinsic asymmetry in nucleosome positioning between lagging and leading strands.
bioRxiv · Sep 18, 2025 · PreprintBiochemistry