Targeted profiling of human extrachromosomal DNA by CRISPR-CATCH
K. L. Hung, J. Luebeck, S. R. Dehkordi, C. Coruh, J. A. Law, W. J. Greenleaf, P. S. Mischel, V. Bafna, H. Y. Chang
This study introduces CRISPR-CATCH, a method to purify and analyze extrachromosomal DNA, revealing hypomethylation and structural details of ecDNA in cancer cells.
bioRxiv · Nov 28, 2021Cancer Biology