Cancer BiologybioRxivPreprint
Dissociation protocols used for sarcoma tissues bias the transcriptome observed in single-cell and single-nucleus RNA sequencing
D. D. Truong, S.-E. Lamhamedi-Cherradi, R. W. Porter…·J. A. Ludwig
The paper shows that dissociation methods bias transcriptomes in sarcoma single-cell and single-nucleus RNA-seq, and demonstrates computational correction of these biases.