Dissociation protocols used for sarcoma tissues bias the transcriptome observed in single-cell and single-nucleus RNA sequencing
D. D. Truong, S.-E. Lamhamedi-Cherradi, R. W. Porter, S. Krishnan, J. Swaminathan, A. Gibson, A. J. Lazar, J. A. Livingston, V. Gopalakrishnan, N. Gordon, N. C. Daw, R. Gorlick, J. A. Ludwig
The paper shows that dissociation methods bias transcriptomes in sarcoma single-cell and single-nucleus RNA-seq, and demonstrates computational correction of these biases.
bioRxiv · Jan 21, 2022 · PreprintCancer Biology