Author

J. F. X. Diffley

Listed by name: these papers carry no ORCID for this author, so different people with the same name may be mixed together.

Biology Disciplines

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Biochemistry· 2 Biophysics· 2 Cell Biology· 1

Signature Assays, Organisms & Methods

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Approach:experimental assay· 5 Research Focus:chromatin & epigenetic regulation· 3 Organism:yeast· 3 Research Focus:cell type annotation· 1 Assay:cryo-EM· 1 Organism:human· 1

Frequent Co-Authors

B. Solano· 2 shared E. van Veen· 2 shared H. Sanchez· 2 shared N. H. Dekker· 2 shared T. van Laar· 2 shared A. Costa· 1 shared A. E. Ikui· 1 shared A. Silva· 1 shared

Activity by Year

2020-2024 · peak 1/yr

Co-Author Collaboration Network

10 top collaborators · 18 mutual co-author links

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2 shared papersBiophysics

B. Solano

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5 papers

Recent·Most cited

MCM Double Hexamer Loading Visualised with Human Proteins

F. Weissmann, J. F. Greiwe, T. Pühringer, T. C. R. Miller, J. F. X. Diffley, A. Costa

The paper characterizes human MCM double hexamer (hDH) loading using biochemical reconstitution and cryo-EM, revealing differences from yeast in assembly mechanisms and identifying a novel intermediate.

bioRxiv · Apr 10, 2024 · PreprintBiochemistry

A chromatinized origin reduces the mobility of ORC and MCM through interactions and spatial constraint

H. Sanchez, Z. Liu, E. van Veen, T. van Laar, B. Solano, J. F. X. Diffley, N. H. Dekker

The paper investigates ORC and MCM dynamics at chromatinized origins in yeast, finding that chromatin reduces MCM mobility while maintaining ORC binding efficiency.

bioRxiv · May 18, 2023Biophysics

Nucleotide binding halts diffusion of the eukaryotic replicative helicase during activation

D. Ramirez Montero, H. Sanchez, E. van Veen, T. van Laar, B. Solano, J. F. X. Diffley, N. H. Dekker

This study reports the first single-molecule activation of CMG reconstituted from purified yeast proteins and quantifies its DNA motion, showing nucleotide binding halts diffusion and enables DNA melting.

bioRxiv · Dec 23, 2022Biophysics

Cdc6 is sequentially regulated by PP2A-Cdc55, Cdc14 and Sic1 for origin licensing in S. cerevisiae

J. Philip, M. Ord, A. Silva, S. Singh, J. F. X. Diffley, D. Remus, M. Loog, A. E. Ikui

This study identifies the sequential regulation of Cdc6 phosphorylation by PP2A-Cdc55, Cdc14, and Sic1 in S. cerevisiae to control origin licensing.

bioRxiv · Sep 14, 2021Cell Biology

Can a nick promote partial genome re-replication?

E. Johansson, J. F. X. Diffley

The paper investigates whether a DNA nick can lead to partial genome re-replication in eukaryotic cells by modeling the repair process with purified proteins.

bioRxiv · Nov 14, 2020 · PreprintBiochemistry