Dynamic action of the Sec machinery during initiation, protein translocation and termination revealed by single molecule fluorescence
T. Fessl, D. Watkins, P. Oatley, W. J. Allen, R. A. Corey, J. Horne, S. A. Baldwin, S. E. Radford, I. Collinson, R. Tuma
This study uses single molecule FRET to analyze Sec machinery dynamics during protein translocation, revealing initiation, transport, and termination phases with specific roles for SecA and ATP hydrolysis.
bioRxiv · Jan 17, 2018Biophysics