I. Collinson

A whole cell luminescence-based screen for inhibitors of the bacterial Sec machinery

The paper develops a high-throughput luminescence-based screen to identify bacterial Sec machinery inhibitors using a split NanoLuc luciferase assay in living cells.

The α-dystroglycan N-terminus is a broad-spectrum antiviral agent against SARS-CoV-2 and enveloped viruses

This study shows that the N-terminal fragment of α-dystroglycan blocks SARS-CoV-2 and other enveloped viruses in cell culture and animal models.

Energy landscape steering mediates dynamic coupling in ATP-driven protein translocation by the bacterial Sec machinery

This study uses single-molecule fluorescence to show that SecYEG dynamically couples with SecA through energy landscape steering, enhancing protein translocation efficiency.

The consequence of ATP synthase dimer angle on mitochondrial morphology studied by cryo-electron tomography

The paper uses cryo-electron tomography to study ATP synthase dimer angles in C. elegans and compares them to yeast, revealing differences in mitochondrial morphology and potential functional implications.

Intercellular Mitochondrial Transfer as a Rescue Mechanism in Response to Protein Import Failure

This study shows that mammalian cells can rescue mitochondrial protein import failure by transferring healthy mitochondria between cells via tunneling nanotubes.

Perturbation of the mitochondrial import machinery by aggregation prone Tau affects organelle morphology and reduces neuronal complexity

This study investigates how TauP301L affects mitochondrial import, morphology, and function in neurons, revealing a link between aggregation-prone proteins and neurodegeneration.

The NanoLuc Assay System for Accurate Real-Time Monitoring of Mitochondrial Protein Import within Intact Mammalian Cells

This paper develops a NanoLuc assay system to monitor mitochondrial protein import in live mammalian cells, enabling kinetic analysis and mechanistic insights into the process.

INTERACTION OF THE PERIPLASMIC CHAPERONE SURA WITH THE INNER MEMBRANE PROTEIN SECRETION (SEC) MACHINERY

The paper investigates how SurA interacts with the SecYEG translocon to protect nascent proteins during their transport across the bacterial envelope.

Rate-limiting transport of positive charges through the Sec-machinery is integral to the mechanism of protein transport

The paper investigates protein transport through the Sec-machinery, finding that arginine diffusion limits transport and PMF contributes via a proton ratchet mechanism.

A bacterial secretosome for regulated envelope biogenesis and quality control

This paper explores the biochemical mechanisms of the bacterial secretosome, focusing on its role in envelope biogenesis and quality control through interactions involving the Sec translocon and associated factors.

Towards a molecular mechanism underlying mitochondrial protein import through the TOM and TIM23 complexes

This study uses a split NanoLuc luciferase assay to analyze the kinetics of mitochondrial protein import through the TIM23MOTOR complex, identifying rate-limiting steps and their dependence on precursor properties.

Inter-membrane association of the Sec and BAM translocons for bacterial outer-membrane biogenesis

The paper investigates the inter-membrane association between Sec and BAM translocons in bacterial outer-membrane biogenesis, showing their interaction ensures efficient OMP maturation.

Refined measurement of SecA-driven protein transport reveals indirect coupling to ATP turnover

This study uses a split luciferase assay to measure SecA-driven protein transport in bacteria, revealing stepwise transport coupled to ATP hydrolysis without a distinct initiation event.

HDX-MS reveals nucleotide-regulated, anti-correlated opening and closure of SecA and SecY channels of the bacterial translocon

This study uses HDX-MS to show that ATP binding causes SecY to open and SecA to close, while ATP hydrolysis reverses this, supporting a Brownian ratchet mechanism for protein translocation.

Structure and dynamics of the central lipid pool and protein components of the bacterial holo-translocon

The paper investigates the structure and dynamics of the bacterial holo-translocon using SANS and molecular dynamics simulations, revealing a central lipid core involved in membrane protein insertion.

A High-Resolution Luminescent Assay For Rapid And Continuous Monitoring Of Protein Translocation Across Biological Membranes

This paper introduces a high-resolution luminescent assay for rapid and continuous monitoring of protein translocation, demonstrating its application in bacterial and mitochondrial systems.

Effect of Sec61 interaction with Mpd1 on Endoplasmic Reticulum-Associated Degradation

The paper identifies Mpd1 as an ERAD factor that interacts with Sec61 and shows that this interaction is required for CPY* ERAD.

Dynamic action of the Sec machinery during initiation, protein translocation and termination revealed by single molecule fluorescence

This study uses single molecule FRET to analyze Sec machinery dynamics during protein translocation, revealing initiation, transport, and termination phases with specific roles for SecA and ATP hydrolysis.

Specific cardiolipin-SecY interactions are required for proton-motive-force stimulation of protein secretion

This study identifies and validates two conserved cardiolipin binding sites on the SecA-SecYEG complex, showing their role in ATP and PMF-driven protein secretion.

ATP-induced asymmetric pre-protein folding: a driver of protein translocation?

The paper uses molecular dynamics simulations to study ATP-driven pre-protein translocation through the SecA-SecYEG complex, showing nucleotide-dependent asymmetry in secondary structure formation.